General Cell Based Assays
ELABSCIENCE Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method) (E-BC-K261-M) 96 tests
Triglycerides (TG) can be hydrolyzed by lipoprotein lipase into glycerol and free fatty acids. Glycerol produces glycerol-3-phosphate and ADP under the catalysis of glycerol kinase (GK). Glycerol-3-phosphate produces hydrogen peroxide under the action of glycerol phosphate oxidase (GPO). In the presence of trinder’s reagent, hydrogen peroxide is catalyzed by peroxidase to produce quinones which is proportional to the content of TG.
ELABSCIENCE Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method) (E-BC-K109-M) 48 Tests
Total cholesterol includes free cholesterol and cholesterol esters. Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce hydrogen peroxide. In the presence of Trinder's reagent, hydrogen peroxide is catalyzed by peroxidase (POD) to produce a red quinoneimine dye, whose color intensity is directly proportional to the TC concentration and can be measured at 510 nm.
Eurofins Discoverx cAMP Hunter eXpress GLP1R CHO-K1 GPCR Assay, 2-Plate Kit. (95-0062E2CP2M)
The cAMP Hunter GLP1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of GLP1R GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP1R, a naturally Gs coupled GPCR. Upon activation of GLP1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Mouse EBI2 (GPR183) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse EBI2 (GPR183) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure mEBI2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
MOLTOX Molecular Toxicology Inc 60-251.4L - Lyophilized NADPH REGENSYS B Plus. Intended for use in EAT testing. Supports 5-30% S9 mix. 40ml refers to final volume of S9 mix/pkg. 140mg of crystalline NADP. Add to A Plus. Store in freezer.
60-251.4L - Lyophilized NADPH REGENSYS B Plus. Intended for use in EAT testing. Supports 5-30% S9 mix. 40ml refers to final volume of S9 mix/pkg. 140mg of crystalline NADP. Add to A Plus. Store in freezer.
CPC Scientific H-Thr-Ser-Gly-Pro-Leu-Gly-Asn-Leu-Ala-Glu-Glu-Leu-Asn-Gly-Tyr-Ser-Arg-Lys-Lys-Gly-Gly-Phe-Ser-Phe-Arg-Phe-NH2 (trifluoroacetate salt) 1MG
An RF-amide peptide ligand for SP9155, an orphan G-protein-coupled receptor with high homology to orexin, neuropeptide FF and CCK receptors. It has been shown to potently activate SP9155 with an EC50 of 7 nM.ONE-LETTER SEQUENCE: TSGPLGNLAEELNGYSRKKGGFSFRF-NH2MOLECULAR FORMULA: C127H195N37O37MOLECULAR WEIGHT:2832.17STORAGE CONDITIONS: -20 5C, CAS REGISTRY NUMBER: [600171-68-4], RESEARCH AREA: PainREFERENCES: N.Chartrel et al., Proc. Natl. Acad. Sci. USA, 100, 15247 (2003); Y.Jiang et al., J. Biol. Chem., 278, 27652 (2003)
Eurofins Discoverx PathHunter Human CRTH2 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human CRTH2 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CRTH2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human AVPR1B β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human AVPR1B β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure AVPR1B (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter™ Human OPRL1 (Opioid) Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human OPRL1 Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure OPRL1 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GPR120 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR120 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR120L (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress AVPR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0588E2CP0M)
The PathHunter AVPR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. vasopressin) based activation of AVPR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AVPR2 and EA-tagged β-Arrestin-2. Activation of AVPR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human & Mouse Total ERK Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Total ERK Detection Kit, 50,000 Assay Points