General Cell Based Assays
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-VASP (Ser157) Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Phospho-VASP (Ser157) Detection Kit, 10,000 Assay Points
Meso Scale Discovery U-PLEX Human ICAM-1 ASY
The Meso Scale Discovery U-PLEX Human ICAM-1 Assay Kit is designed to quantitatively measure Intercellular Adhesion Molecule 1 (ICAM-1) in human serum plasma and cell culture supernatants using an electrochemiluminescent detection method This assay kit includes a 96-well plate coated with capture antibody detection antibody conjugated to an electrochemiluminescent label calibrator and buffer for sample dilution The assay has a detection range of 78-50 000 pg/mL and exhibits no cross-reactivity with other adhesion molecules This product can aid in understanding ICAM-1 levels related to inflammatory and immune responses
Cytek Biosciences Inc MAPK Activation Amnis
Product Name: Muse MAPK Activation Dual Detection Kit
Cytek Catalog Number: MCH200104
Product Class: Guava Muse Reagent
Type: Muse
Size: 50 Tests
ABclonal Technology Actin (plant specific) Rabbit pAb
Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Eurofins Discoverx PathHunter Human GPR22 β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human GPR22 β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure GPR22 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Enzo Life Sciences FLUOR DE LYS SIRT5 fluorometric drug discovery assay kit (100 tests)
The SIRT5 Fluorometric Drug Discovery Kit is a complete assay system designed to measure the lysyl desuccinylase activity of the recombinant human SIRT5 included in the kit. A black 96-well microplate is packaged with the kit, but it should be noted that reagents of the FLUOR DE LYS system have also been successfully employed in other formats, including cuvettes and 384-well plates. The SIRT5 Fluorometric Activity Assay is based on the unique FLUOR DE LYS-Succinyl Substrate/Developer combination. The FLUOR DE LYS-Succinyl Substrate, which comprises a single lysine residue, Nε-succinylated on its side-chain, is first incubated with human recombinant SIRT5 together with the cosubstrate NAD+. Desuccinylation of FLUOR DE LYS-Succinyl sensitizes it so that, in the second step, treatment with the FLUOR DE LYS Developer produces a fluorophore. Alternative name: Sirtuin 5 fluorescent assay kit. Applications: Fluorescent detection, HTS Cell-based assays, Activity assay.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Multiplex Human & Mouse Phospho-SMAD2 (Ser465/467 & Total) Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Multiplex Human & Mouse Phospho-SMAD2 (Ser465/467 & Total) Detection Kit, 50,000 Assay Points
Cayman Chemical SIRT1 FRET-Based Screening Assay Kit, 96 Well
Human SIRT1 is the homolog of yeast Sir2 and has been shown to regulate the activity of the p53 tumor suppressor and inhibit apoptosis. Cayman’s SIRT1 FRET-based Screening Assay provides a convenient fluorescence-based method for screening SIRT1 inhibitors or activators. The procedure requires only two easy steps, both performed in the same microplate. In the first step, the substrate, which is coupled to the fluorophore and quencher, is incubated with human recombinant SIRT1 along with its cosubstrate NAD+. Deacetylation sensitizes the substrate such that treatment with the developer in the second step results in the separation of the quencher and fluorophore. The resulting fluorescence is analyzed using an excitation wavelength between 335-345 nm and emission wavelength between 440-465 nm.
Eurofins Discoverx PathHunter eXpress mCXCR3 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0553E2MCP0M)
The PathHunter mCXCR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL11/I-TAC) based activation of mCXCR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCXCR3 and EA-tagged β-Arrestin-2. Activation of mCXCR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress HEK 293 BRDT(1,2) Bromodomain Assay, 10-plate Kit. (96-0046E1CP7L)
The InCELL Hunter eXpress HEK 293 BRDT(1,2) Bromodomain Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of BRDT(1,2) via detection of BRDT(1,2) levels. This product uses EFC technology. The cell line included overexpresses BRDT(1,2) target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Cambinol, SIRT1/2 inhibitor, 5MG
MW 360.4 Da, Purity >98%. SIRT1/2 inhibitor (IC₅₀ values are 56 and 59 μM at SIRT1 and SIRT2 respectively). Class III HDAC inhibitor, with no inhibition of class I and II. Promotes G₂ cell cycle arrest. Antitumour activity in vitro and in vivo.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress CX3CR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0956E2CP0L)
The PathHunter CX3CR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CX3CL1/fractalkine) based activation of CX3CR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CX3CR1 and EA-tagged β-Arrestin-2. Activation of CX3CR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter SSTR2 Bioassay Kit, 2-Plate. (93-0181Y2-00067)
The PathHunter SSTR2 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. somatostatin 28) based activation of SSTR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SSTR2 and EA-tagged β-Arrestin-2. Activation of SSTR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Meso Scale Discovery U-PLEX Human IL-17D ASY
The Meso Scale Discovery U-PLEX Human IL-17D Assay Kit is a multiplex immunoassay for measuring IL-17D in human serum plasma and cell culture supernatant It uses electrochemiluminescence detection offering high sensitivity a wide dynamic range and low sample volume needs The kit includes a 96-well plate with capture antibodies detection antibodies with electrochemiluminescent labels calibrators and diluents It quantifies IL-17D with minimal hands-on time using an MSD instrument providing a simple rapid method to measure IL-17D important for immune regulation